Ek Se Badhkar Ek begins with the story of a gardener's son Rahil (Suniel Shetty) who inherits a windfall of Rs 4 billion! His Anand (Shekhar Suman) commits a blunder by accidentally inserting a term in the will according to which Rahul must prove himself to be the biggest don of the city.
Thus begins a chronicle of mishaps wherein the two attempt to achieve their goal. An ambitious police officer Kanchan (Raveena Tandon) turns out to be Anand's new neighbour. She wants to catch the biggest don in the city and he wants to become the biggest don in the city.
After their initial scuffle, they both team up to help each other. She will get him an entry into the criminal world and he must help her by providing information about the underworld to enable her to put them behind bars. They also come to an agreement that their relationship will be purely professional with no romance coming in between. This goes well with Anand as he falls in love with Kanchan. But in the process Kanchan starts getting attracted to Rahul.
Now Kanchan gets a clue that takes her to the biggest don of city, Jindal who plans to kidnap a very famous nuclear scientist, Krishnamurthy. Tracy (Isha Koppikar) is also involved in this kidnap. Now Rahul gets attracted to Tracy who makes use of him to successfully kidnap the scientist. However Rahul ends up kidnapping Jindal assuming him to be the scientist.